屠宰场需要什么手续:Stripping buffer recipe

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Mild strippingBuffer, 1 L
15 g glycine1 g SDS10 ml Tween 20Adjust pH to 2.2Bring volume up to 1 L with ultrapure water.
Membrane incubationUse a volume that will cover the membrane. Incubate at room temperature for 5-10 minutes.Discard buffer.5-10 minutes fresh stripping buffer.Discard buffer.10 minutes PBS10 minutes PBS5 minutes TBST5 minutes TBST
Ready for blocking stage.
Harsh strippingPrepare buffer and strip membranes under a fumehood.Buffer, 0.1 L
20 ml SDS 10%12.5 ml Tris HCl pH 6.8 0.5 M67.5 ml ultra pure waterAdd 0.8 ml ?-mercaptoethanol under the fumehood.
ProcedureWarm the buffer to 50°C.Add the buffer to a small plastic box which has a tight lid. Use a volume that will cover the membrane.Add the membrane. Incubate at 50°C for up to 45 minutes with some agitation.Dispose of the solution as required for ?-mercaptoethanol based buffers.Rinse the membrane under running water tap for 1-2 hours.Traces of ?-mercaptoethanol will damage the antibodies. Wash extensively for 5 minutes in TBST.

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